A reproducible source-linked case · PRJEB70964
From selection history to reported evidence
13Linked constructs
27Archived round objects
351Count observations
2Reported measurements
Selection history
Blue encodes the percentage of exact observations within each archived object. A white cell is a file-scoped zero; unavailable archive rounds are omitted.
Read percentage · logarithmic scale: 10−6% 100%
Reported construct · F005
- Variable region
- 35 nt
- Full construct
- 105 nt
- Reported chemistry
- RNA-2'FPy
- Source identity
- SYN-S6-R9 ↔ SYN-S2-R877
Sequence correspondence alone does not establish complete chemical identity.
Exact observations by archived object
| Archive object | Exact count | All archived reads | Read percentage |
|---|---|---|---|
| ERR12353593 | 40 | 9,502,133 | 0.000420958% |
| ERR12353594 | 5 | 8,185,266 | 6.10854e-05% |
| ERR12353595 | 3 | 9,587,680 | 3.12902e-05% |
| ERR12353596 | 1 | 9,905,602 | 1.00953e-05% |
| ERR12353597 | 11 | 8,960,058 | 0.000122767% |
| ERR12353598 | 51 | 7,340,945 | 0.000694733% |
| ERR12353599 | 29 | 9,072,306 | 0.000319654% |
| ERR12353600 | 99 | 8,887,432 | 0.00111393% |
| ERR12353601 | 675 | 8,643,029 | 0.00780976% |
| ERR12353602 | 2,097 | 7,254,346 | 0.0289068% |
| ERR12353603 | 6,799 | 8,903,218 | 0.0763656% |
| ERR12353604 | 18,000 | 8,994,132 | 0.20013% |
| ERR12353605 | 80,450 | 8,135,966 | 0.988819% |
| ERR12353606 | 117,698 | 5,960,615 | 1.97459% |
| ERR12353607 | 76 | 10,888,889 | 0.000697959% |
| ERR12353608 | 4 | 12,203,941 | 3.27763e-05% |
| ERR12353609 | 36 | 11,026,739 | 0.000326479% |
| ERR12353610 | 42 | 11,238,722 | 0.000373708% |
| ERR12353611 | 22 | 8,729,608 | 0.000252016% |
| ERR12353612 | 17 | 10,436,126 | 0.000162896% |
| ERR12353613 | 104 | 9,751,625 | 0.00106649% |
| ERR12353614 | 448 | 10,255,907 | 0.00436821% |
| ERR12353615 | 2,696 | 13,605,485 | 0.0198155% |
| ERR12353616 | 7,951 | 14,925,955 | 0.0532696% |
| ERR12353617 | 16,803 | 15,225,941 | 0.110358% |
| ERR12353618 | 38,717 | 11,569,086 | 0.334659% |
| ERR12353619 | 64,056 | 12,217,417 | 0.524301% |
Source-reported apparent affinity
No quantitative affinity value was extracted for this construct in this case.
Data & reproducibility
Complete case (JSON)Figure 3 (PDF)Version manifest
| archive-rounds.tsv | SHA-256 8d58ec39596b8b29b7a7781b4feea426262f2001471c1c56ce43ec11ed34e5bc |
| construct-links.tsv | SHA-256 8e314b73c212d4fc198af18916f06ac321032fd83054cc10ef2d85d938dff9f8 |
| published-percentage-crosscheck.tsv | SHA-256 40fac50d6c8e144ed0c0082b2eb47a355c1f3fd6f01b412eb882980da3865dc3 |
| read-frequency-source-data.tsv | SHA-256 7a65345864269156e4d0f46006ee2b2d60906c6827b401b7d85c747ab676c0de |
| reported-kd-source-data.tsv | SHA-256 0fec9450b608b9e01eb9ceae6995cebb4e22c168073cd7e0be7918e0b56566c0 |
Sources and reuse
Bouvier-Muller et al., Nucleic Acids Research (2024), doi:10.1093/nar/gkae544; ENA PRJEB70964. · ENA PRJEB70964
Source metadata were selected and typed; archive counts and source correspondence were independently recalculated. Publisher artwork and nucleotide strings are not included.
Interpretation
- Read frequency and binding affinity use different denominators and experimental contexts.
- A zero means no exact correspondence in one archived object; omitted rounds are not zero observations.
- Source substring correspondence does not establish complete chemical identity.
- The two apparent Kd values refer to the reported full assay constructs; F30 maps to same-paper F030.
- This case is separately scoped and is not added to the core construct or measurement totals.