A reproducible source-linked case · PRJEB70964
From selection history to reported evidence
13Linked constructs
27Archived round objects
351Count observations
2Reported measurements
Selection history
Blue encodes the percentage of exact observations within each archived object. A white cell is a file-scoped zero; unavailable archive rounds are omitted.
Read percentage · logarithmic scale: 10−6% 100%
Reported construct · F124
- Variable region
- 35 nt
- Full construct
- 105 nt
- Reported chemistry
- RNA-2'FPy
- Source identity
- SYN-S6-R15 ↔ SYN-S2-R2230
Sequence correspondence alone does not establish complete chemical identity.
Exact observations by archived object
| Archive object | Exact count | All archived reads | Read percentage |
|---|---|---|---|
| ERR12353593 | 1 | 9,502,133 | 1.0524e-05% |
| ERR12353594 | 0 | 8,185,266 | 0% |
| ERR12353595 | 0 | 9,587,680 | 0% |
| ERR12353596 | 0 | 9,905,602 | 0% |
| ERR12353597 | 0 | 8,960,058 | 0% |
| ERR12353598 | 0 | 7,340,945 | 0% |
| ERR12353599 | 2 | 9,072,306 | 2.20451e-05% |
| ERR12353600 | 7 | 8,887,432 | 7.87629e-05% |
| ERR12353601 | 47 | 8,643,029 | 0.000543791% |
| ERR12353602 | 68 | 7,254,346 | 0.000937369% |
| ERR12353603 | 204 | 8,903,218 | 0.00229131% |
| ERR12353604 | 1,106 | 8,994,132 | 0.0122969% |
| ERR12353605 | 2,575 | 8,135,966 | 0.0316496% |
| ERR12353606 | 2,055 | 5,960,615 | 0.0344763% |
| ERR12353607 | 6 | 10,888,889 | 5.5102e-05% |
| ERR12353608 | 0 | 12,203,941 | 0% |
| ERR12353609 | 1 | 11,026,739 | 9.06886e-06% |
| ERR12353610 | 0 | 11,238,722 | 0% |
| ERR12353611 | 2 | 8,729,608 | 2.29105e-05% |
| ERR12353612 | 2 | 10,436,126 | 1.91642e-05% |
| ERR12353613 | 0 | 9,751,625 | 0% |
| ERR12353614 | 4 | 10,255,907 | 3.90019e-05% |
| ERR12353615 | 10 | 13,605,485 | 7.34998e-05% |
| ERR12353616 | 22 | 14,925,955 | 0.000147394% |
| ERR12353617 | 34 | 15,225,941 | 0.000223303% |
| ERR12353618 | 23 | 11,569,086 | 0.000198806% |
| ERR12353619 | 4 | 12,217,417 | 3.27401e-05% |
Source-reported apparent affinity
| Construct | Kd,app ± SD (nM) | Assay context |
|---|---|---|
| F124 | 8.8 ± 1.3 | Membrane-retention assay of extended construct hybridized with labelled oligonucleotide |
F-type α-synuclein fibrils · n = 3 independent experiments · doi:10.1093/nar/gkae544
Data & reproducibility
Complete case (JSON)Figure 3 (PDF)Version manifest
| archive-rounds.tsv | SHA-256 8d58ec39596b8b29b7a7781b4feea426262f2001471c1c56ce43ec11ed34e5bc |
| construct-links.tsv | SHA-256 8e314b73c212d4fc198af18916f06ac321032fd83054cc10ef2d85d938dff9f8 |
| published-percentage-crosscheck.tsv | SHA-256 40fac50d6c8e144ed0c0082b2eb47a355c1f3fd6f01b412eb882980da3865dc3 |
| read-frequency-source-data.tsv | SHA-256 7a65345864269156e4d0f46006ee2b2d60906c6827b401b7d85c747ab676c0de |
| reported-kd-source-data.tsv | SHA-256 0fec9450b608b9e01eb9ceae6995cebb4e22c168073cd7e0be7918e0b56566c0 |
Sources and reuse
Bouvier-Muller et al., Nucleic Acids Research (2024), doi:10.1093/nar/gkae544; ENA PRJEB70964. · ENA PRJEB70964
Source metadata were selected and typed; archive counts and source correspondence were independently recalculated. Publisher artwork and nucleotide strings are not included.
Interpretation
- Read frequency and binding affinity use different denominators and experimental contexts.
- A zero means no exact correspondence in one archived object; omitted rounds are not zero observations.
- Source substring correspondence does not establish complete chemical identity.
- The two apparent Kd values refer to the reported full assay constructs; F30 maps to same-paper F030.
- This case is separately scoped and is not added to the core construct or measurement totals.