NDM-1: registration proposal
PRJNA830749 · Data group 2633
Material proposal prepared; reference correspondence unresolved
The source reports His-tagged NDM-1. Its provenance wording does not establish natural source species or expression host. C7C422 came from an unapproved automatic name match.
Current retained data
10,000Retained candidate records
4Retained round records
40,000Sequence–round observations
Source-reported material
- Assayed protein source species
- Not established
- Material
- N-terminally hexa-HIS-tagged NDM-1
- Expression host
- Not established
- Reported provenance
- NDM-1 (derived from E. coli)
- Source-reported supplier
- Hölzel Diagnostica Handels GmbH
Reference and relationship
- Reference accession
- C7C422
- Reference database
- UniProtKB
- Reference organism
- Klebsiella pneumoniae
- Reference taxonomy ID
- 573
- Reference scope
- external_name_correspondence_not_exact_construct_identity
The evidence and limitations below define how the reference relates to the actual experimental material.
Sources and evidence positions
- Author-reported tagged material and ambiguous provenanceMethods > Target preparation > first paragraph, first sentence
- External reference species differs from unresolved assay provenanceReference identity metadata only; not an assay construct match
Field corrections
| Field | Original value | Current value | Basis |
|---|---|---|---|
| registry_relation | entry_absent | source_named_material_registration_proposal | Primary article material name, explicit project linkage, and 677-row equivalence audit. |
| assayed_protein_source_organism | Klebsiella pneumoniae | Not established | Author wording derived from E. coli is ambiguous; Klebsiella belongs to the external reference entry. |
| reported_expression_host | Not established | Not established | No explicit expression-host statement for the purchased NDM-1 material was identified. |
| reference_accession_relation | C7C422 without qualification | C7C422 external reference candidate; not author-reported or construct-verified | Official reference metadata plus unapproved free-text resolution provenance. |
Remaining evidence gaps
- Exact purchased construct accession or supplier lot/catalogue identity is not reported in the reviewed material paragraph.
- Natural protein source species and expression/preparation host are not distinguished by the author phrase derived from E. coli.
- Exact assayed-construct equivalence to C7C422 has not been established.